Main profile

Manjusha Dixit

School: 

Biological Sciences

Designation: 

Associate Professor

Education: 

• Post-doctoral Fellow- 2007-2009: John A. Moran Eye Center, University of Utah, Salt Lake City, Utah, USA, Broad area- Genetics of eye diseases
• Post-doctoral Fellow- 2006-2007: Center for Genetic Medicine Research, Children’s National Medical Center, Washington DC, USA, Broad area- Role of Pitx1 in FSHD
• Doctoral- 2002-2006: Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India, Broad area-  Medical Genetics
• Masters- 2001: School of Studies in Biochemistry, Jiwaji University, Gwalior, India, Broad area-  Biochemistry                      

Specialisation: 

Tumour Angiogenesis Regulation and Cancer Genetics

Awards: 

• Editorial Board Member, BMC Cancer, 2020- present
• Editorial Board Member, Scientific Reports, 2019- present

Publications: 

Academic Publications 

 

1. R Khan, A Palo, M Dixit. Role of FRG1 in predicting the overall survivability in cancers using multivariate based optimal model. Scientific Reports, 2021 (Accepted)
2. D Kumar, SA Patel, R Khan, S Chawla, N Mohapatra, M Dixit. IQ motif-containing GTPase-activating protein 2 inhibits breast cancer angiogenesis by suppressing VEGFR2-AKT signalling. Molecular Cancer Research, (Accepted)
3. KK Sinha, J Vinay, S Parida, SP Singh, M Dixit. Association and Functional Significance of Genetic Variants Present in Regulatory Elements of SERPINB5 Gene in Gallbladder Cancer. Gene, 2021, 1145989 

4. D Kumar, SA Patel, MK Hassan, N Mohapatra, N Pattanaik, M Dixit. Reduced IQGAP2 expression promotes EMT and inhibits apoptosis by modulating the MEK-ERK and p38 signaling in breast cancer irrespective of ER status. Cell death & disease, 2021, 12 (4), 1-14

5. J Vinay, Debakanta Mishra, Dinesh Meher, Sashibhusan Dash, Kusumbati Besra, Niharika Pattnaik, Shivaram Prasad Singh, Manjusha Dixit. Genetic association of MMP14 promoter variants and their functional significance in gallbladder cancer pathogenesis. Journal of Human Genetics,  2021, 1-10

6. B Mahananda, J Vinay, A Palo, A Singh, SK Sahu, SP Singh, M Dixit. SERPINB5 Genetic Variants rs2289519 and rs2289521 are Significantly Associated with Gallbladder Cancer Risk. DNA and cell biology, 2021, 40 (5), 706-712

7. A Bollu, MK Hassan, M Dixit, NK Sharma. The 2′-caged-tethered-siRNA shows light-dependent temporal controlled RNAi activity for GFP gene into HEK293T cells. Bioorganic & Medicinal Chemistry, 2021, 30, 115932             

8. MK Hassan, D Kumar, SA Patel, M Dixit. EEF1A2 triggers stronger ERK mediated metastatic program in ER negative breast cancer cells than in ER positive cells. Life Sciences 2020, 262, 118553

9. Hassan MK, Kumar D, Patel SA, Pattanaik N, Mohapatra N, Dixit M. Expression pattern of EEF1A2 in brain tumors: Histological analysis and functional role as a promoter of EMT. Life Sci. 2020 Apr 1;246:117399. doi: 10.1016/j.lfs.2020.117399. Epub 2020 Feb 4. PubMed PMID: 32032648.

10. Tiwari A, Mukherjee B, Hassan MK, Pattanaik N, Jaiswal AM, Dixit M. Reduced FRG1 expression promotes prostate cancer progression and affects prostate cancer cell migration and invasion. BMC Cancer. 2019;19(1):346. doi: 10.1186/s12885-019-5509-4. PMID:30975102. ISSN 1471-2407

11. Naik, M.; Brahma, P.; Dixit, M. A Cost-Effective and Efficient Chick Ex-Ovo CAM Assay Protocol to Assess Angiogenesis. Methods Protoc. 2018, 1, 19.

12.  Hassan MK, Kumar D, Naik M, Dixit M. The expression profile and prognostic significance of eukaryotic translation elongation factors in different cancers. PLoS One. 2018 Jan 17;13(1):e0191377. doi: 10.1371/journal.pone.0191377. eCollection 2018. PubMed PMID: 29342219; PubMed Central PMCID: PMC5771626.

13. Kumar D, Hassan MK, Pattnaik N, Mohapatra N, Dixit M. Reduced expression of IQGAP2 and higher expression of IQGAP3 correlates with poor prognosis in cancers. PLoS One. 2017 Oct 26;12(10): e0186977. doi: 10.1371/journal.pone.0186977. eCollection 2017. PubMed PMID: 29073199; PubMed Central PMCID: PMC5658114.

14. Tiwari A, Mukherjee B, Dixit M. MicroRNA Key to Angiogenesis Regulation: miRNA Biology and Therapy. Curr Cancer Drug Targets. 2017 Jun 30. doi: 10.2174/1568009617666170630142725. PubMed PMID: 28669338.

15. Tiwari A, Pattnaik N, Mohanty Jaiswal A, Dixit M. Increased FSHD region gene1 expression reduces in vitro cell migration, invasion, and angiogenesis, ex vivo supported by reduced expression in tumors. Biosci Rep. 2017 Oct 27;37(5). pii: BSR20171062. doi: 10.1042/BSR20171062. Print 2017 Oct 31. PubMed PMID: 28947680; PubMed Central PMCID: PMC5665614.

16. Arman Kunwar Hansda, Ankit Tiwari, Manjusha Dixit. Current Status and Future Prospect of FSHD Region Gene 1. J Biosci. 2017 Jun;42(2):345-353.

17. Gade CR, Dixit M, Sharma NK. Dideoxy nucleoside triphosphate (ddNTP)analogues: Synthesis and polymerase substrate activities of pyrrolidinyl nucleoside triphosphates (prNTPs). Bioorg Med Chem. 2016 Sep 15;24(18): 4016-22.doi: 10.1016/j.bmc.2016.06.043.

18. Deepa Kumari, Ankit Tiwari, Mahasweta Choudhury, Abhishek Kumar, Aparna Rao, Manjusha Dixit. A novel KERA mutation in a case of autosomal recessive cornea plana with primary angle closure glaucoma. J Glaucoma. 2016 Feb;25(2):e106-9.

19. Tiwari A, Gade CR, Dixit M, Sharma NK. Methylene Salicylicacidyl Hexamer (MSH) Has DNAse Activity. Appl Biochem Biotechnol. 2015 Jul;176(6):1791-800.

20. Subhransu Sekhar Sahoo, Sebaranjan Biswal, and Manjusha Dixit. Distinctive Mutation Spectrum of the HBB Gene in an Urban Eastern Indian Population. Hemoglobin 2014, 38: 33-38.

21. Sachchida N. Pandey, Jennifer Cabotage, Rongye Shi, Manjusha Dixit, Margret Sutherland, Jian Liu, Stephanie Muger, Scott Q. Harper, Kanneboyina Nagaraju,  and Yi-Wen Chen. Conditional over-expression of PITX1 causes skeletal muscle dystrophy in mice. Biology Open 2012, 1:629-639.

22.  Sharma, N.K., P. Lunawat and M. Dixit. Binding studies of natural product berberine with DNA G-quadruplex. Am. J. Biochem. Biotechnol.2011, 7: 130-134.

23. Yang Z, Tong Z, Chen Y, Zeng J, Lu F, Sun X, Zhao C, Wang K, Davey L, Chen H, London N, Muramatsu D, Salasar F, Carmona R, Kasuga D, Wang X, Bedell M, Dixit M, Zhao P, Yang R, Gibbs D, Liu X, Li Y, Li C, Li Y, Campochiaro B, Constantine R, Zack DJ, Campochiaro P, Fu Y, Li DY, Katsanis N, Zhang K. Genetic and functional dissection of HTRA1 and LOC387715 in age-related macular degeneration. PLoS Genet. 2010 Feb 5;6(2):e1000836 (Name misspelled).

24. Gibbs D, Yang Z, Constantine R, Ma X, Camp NJ, Yang X, Chen H, Jorgenson A, Hau V, Dewan A, Zeng J, Harmon J, Buehler J, Brand JM, Hoh J, Cameron DJ, Dixit M, Tong Z, Zhang K. Further mapping of 10q26 supports strong association of HTRA1 polymorphisms with age-related macular degeneration. Vision Res. 2008 Feb;48(5):685-689.

25. Dixit M, Ansseau E, Tassin A, Winokur S, Shi R, Qian H, Sauvage S, Mattéotti C, van Acker AM, Leo O, Figlewicz D, Barro M, Laoudj-Chenivesse D, Belayew A, Coppée F, Chen YW. DUX4, a candidate gene of facioscapulohumeral muscular dystrophy, encodes a transcriptional activator of PITX1. Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18157-62.

26. Dixit M, Choudhuri G, Saxena R, Mittal B. Association of apolipoprotein A1-C3 gene cluster polymorphisms with gallstone disease. Can J Gastroenterol. 2007 Sep;21(9):569-75.

27. Pandey SN, Srivastava A, Dixit M, Choudhuri G, Mittal B. Haplotype analysis of signal peptide (insertion/deletion) and XbaI polymorphisms of the APOB gene in gallbladder cancer. Liver Int. 2007 Sep;27(7):1008-15.

28. Srivastava A, Pandey SN, Dixit M, Choudhuri G, Mittal B. Cholecystokinin receptor A gene polymorphism in gallstone disease and gallbladder cancer. J Gastroenterol Hepatol. 2008 Jun;23(6):970-5.

29. Dixit M, Choudhuri G, Mittal B. Association of lipoprotein receptor, receptor-associated protein, and metabolizing enzyme gene polymorphisms with gallstone disease: A case-control study. Hepatol Res. 2006 Sep;36(1):61-9.

30. Dixit M, Choudhuri G, Keshri LJ, Mittal B. Association of low density lipoprotein receptor related protein-associated protein (LRPAP1) gene insertion/deletion polymorphism with gallstone disease. J Gastroenterol Hepatol. 2006 May;21(5):847-9.

31. Dixit M, Choudhuri G, Mittal B. Association of APOE-C1 gene cluster polymorphisms with gallstone disease. Dig Liver Dis. 2006 Jun;38(6):397-403.

32. Pandey SN, Dixit M, Choudhuri G, Mittal B. Lipoprotein Receptor Associated Protein (LRPAP1) Insertion/Deletion Polymorphism: Association with Gallbladder Cancer Susceptibility. Int J Gastrointest Cancer. 2006;37(4):124-8.

33. Dixit M, Bhattacharya S, Mittal B. Association of CETP TaqI and APOE polymorphisms with type II diabetes mellitus in North Indians: a case control study. BMC Endocr Disord. 2005 Jul 1;5:7.

34. Dixit, Manjusha; Mittal, B. Frequencies of CETP gene TaqI B and D442G polymorphisms in North Indian Population. Current Science 2005, 88, 1973-1977.

35. Agarwal, G. S.; Kamboj, D. V.; Alam, S. I.; Dixit, Manjusha; Singh, L. Environmental detection of bacillus anthracis spores. Current science 2002 83, 697.

 

Responsibilities: 

-Member secretary Institution Ethics committee, NISER

Research: 

Dr. Dixit's research interest is to understand the genetics and molecular biology of various human genetic diseases, especially cancer.

Abnormal growth of new blood vessels plays an important role in many diseases, including cancer. To treat cancer, various potential anti-angiogenesis drugs have been tested with limited success. Blocking just one regulatory pathway may not be enough. Till date, all these angiogenic-switch regulatory molecules and their mechanism are not known. In order to do that, my research group is interested towards the validation and elucidation of the molecular mechanism of putative angiogenic regulators. We are also interested in finding out the role and molecular mechanism of these newly identified angiogenesis regulators, in tumorigenesis and tumor angiogenesis. We started with FRG1 (a putative angiogenic regulator) and further research led to identification of EEF1A2 and IQGAP2, as its interacting partners. Currently, we have established FRG1 to have reduced expression in cancer and its effect on various properties of cells (proliferation, migration and invasion). We found that IQGAP2 expression is also reduced but the expression of EEF1A2 is increased in tumor tissues. We discovered the mechanism of, how FRG1 and IQGAP2 behave as tumor suppressor genes but EEF1A2 behaves as an oncogene. Our data suggests that all three genes may impart tumorigenic potential by affecting angiogenesis.

Another major area of our research includes understanding molecular mechanism of gallbladder cancer and establishment of genetic risk factors in Indian population. So far, we have
found that SNPs present in MMP14 affect it’s expression and might be responsible for altered risk in the population of Odisha.

Teaching: 

Dr. Dixit regularly offers two theory and two practical courses in odd or even semeter.

• Genetics (B205)
• Genetic Engineering (B401)
• Laboratory- Genetics (B244)
• Laboratory- Genetic Engineering (B441)

Contacts: 

Office:

Tel 0674-2304195, 0674-2304196

Email: manjusha@niser.ac.in

Fax: 0674-2302436

School of Biological Sciences, National Institute of Science Education and Research

PO- Bhimpur-Padanpur, Via- Jatni, District:- Khurda, Odisha

PIN- 752050

Phone No.: 

+91-674-2304195

Room No.: 

204

Mail ID: 

manjusha@niser.ac.in

Personal Page: 

Cancer Genetics and Vasculature Research Group

Group Members: 

Ph.D

• Talina Mohapatra (Dec 2020- Continuing)

• Soham Choudhury (Dec 2020- Continuing)

• Ananya Palo (July 2018- Continuing)

• Saket Avadhbhai Patel (Jan 2018- Continuing)

• Vinay J. (July 2016- Continuing)

• Bratati Mukherjee (July 2016- Continuing)

• Dinesh Kumar (July 2014- 2020)- Currently in Shah Lab, Center for Cancer Research, Department of Chemistry, Purdue University, USA

• Md. Khurshid Hassan (July 2013- 2020)- Corrently in Nagireddy Lab, Molecular & Cellular Biology, Baylor College of Medicine, USA

• Ankit Tiwari (completed 2018)- Currently in Malhotra Lab Centre de Regulació Genòmica  ICREA Barcelona, Spain

 

M.Sc

·       Rehan Khan (2017- continuing)

·       Biswashree Mahanta (2015- 2020)

·       Kirti Kumari Sinha (2014-2019)

·       Neha Ramani (2014-2019)

·       Monali Naik (2013- 2018)

·       Pratush Brahma (2013- 2018)

·       Arman Kunwar Hansda (2011-2016) 

·       Rishikesh Ramarao Sarode (2010-15)

·       Deepa Kumari (2009-2014)

·       Subhransu Sekhar Sahoo (2007-2012)